Mass cytometry papers in diabetes (from the start of CyTOF till now)
You will find the list of papers of mass cytometry (CyTOF) investigating diabetes.
We also propose other reviews, one about mass cytometry data in blood cancers, the other in solid tumors. You might also be interested by the papers using mass cytometry to study COVID. There is a recent review about mass cytometry and type 1 diabetes.
13 November 2020
Diance C. Saunders
Materials
Human tissues
Number of markers
Variable
Online imaging resource containing datasets linking imaging data with clinical data to facilitate advances in the understanding of diabetes, pancreatitis, and pancreatic cancer
Pancreatlas: Applying an Adaptable Framework to Map the Human Pancreas in Health and Disease
Patterns
Materials
Number of markers
15 June 2020
Martin Eichmann
Materials
PBMC from 59 individuals (placebo n = 19, abatacept n = 40)
Number of markers
32 markers
Mass cytometry showed significant association between TCM and β-cell function. Abatacept treatment significantly alters the frequencies of a majority of CD4+ conventional and regulatory T cell subsets but not CD8+ T cells.
Costimulation Blockade Disrupts CD4 + T Cell Memory Pathways and Uncouples Their Link to Decline in β-Cell Function in Type 1 Diabetes
Journal of Immunology
21 February 2020
Louise Magnusson
Materials
44 PBMC (including 15 healthy donors)
Number of markers
32 markers
Mass cytometry comparison of PBMC from patients with new-onset (N-T1D) and long-standing type 1 diabetes, Hashimoto's thyroiditis (HT), Graves' disease and autoimmune Addison's disease (AD), as well as healthy controls. CD56dimCD69+HLA-DR- NK cells were reduced and CD8+ TEMRA cells were reduced in the N-T1D group.
Mass Cytometry Studies of Patients With Autoimmune Endocrine Diseases Reveal Distinct Disease-Specific Alterations in Immune Cell Subsets
Frontiers in Immunology
02 January 2020
Alice E Wiedeman
Materials
66 islets human including 20 controls
Number of markers
22 markers
Study of the phenotype and function of autoreactive CD8+ T cells. influence disease progression with application of new bioinformatic method DISCOV-R. Activated islet-specific CD8+ memory T cells were prevalent in subjects with T1D who experienced rapid loss of C-peptide.
Autoreactive CD8+ T cell exhaustion distinguishes subjects with slow type 1 diabetes progression
Journal of Clinical Investigation
24 November 2019
Jessica Suwandi
Materials
PBMC and in vitro stimulation
Number of markers
35 markers
Identification of a surface-based T cell signature of tolerogenic modulation (specifically tolerogenic dendritic cells, Tol DCs) by CyTOF. TolDCs induced suppressive T cells mostly CD45RA+CCR7+. T cell cultures stimulated by tolDC further contained memory-like CD45RA-CCR7- T cells expressing regulatory markers Lag-3, CD161 and ICOS.
Multidimensional analyses of proinsulin peptide-specific regulatory T cells induced by tolerogenic dendritic cells
Journal of Automimmunity
03 May 2019
Hugo Barcenilla
Materials
9 high risk individuals versus 9 controls
Number of markers
33 markers
CyTOF identified an effector memory Treg subset expressing HLA-DR, CCR4, CCR6, CXCR3, and GATA3 that was increased in the high-risk group. for type 1 diabetes. This was correlated also with increase of other NK subsets defined by CD16+ CD8+ CXCR3+ and CD16+ CD8+ CXCR3+ CD11c+. These markers might be predictive of type 1 diabetes
Mass Cytometry Identifies Distinct Subsets of Regulatory T Cells and Natural Killer Cells Associated With High Risk for Type 1 Diabetes
Frontiers in Immunology
05 March 2019
Nicolas Damond
Materials
12 islets human donors
Number of markers
35 markers
Analysis of 1,581 islets from 12 human donors (8 with type 1 diabete), using imaging mass cytometry (IMC). Pseudotime analysis of islets through T1D progression from snapshot data to reconstruct the evolution of β cell loss and insulitis revealed that β cell destruction is preceded by a β cell marker loss and by recruitment of cytotoxic and helper T cells.
A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry
Cell Metabolism
05 March 2019
Yue J Wang
Materials
12 islets T1D donors and 6 controls
Number of markers
33 markers
Islet cells show changes in protein expression during T1D progression and immune cells are higly proliferative in type 1 diabetes
Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes
Cell Metabolism
08 August 2018
Sandra Laban
Materials
3 islet type 1 diabetes patients
Number of markers
33 markers
Mass cytometry description of autoantigen- (pre-proinsulin), neoantigen- (insulin-DRIP) and virus- (cytomegalovirus) reactive CD8 T-cells.
Heterogeneity of circulating CD8 T-cells specific to islet, neo-antigen and virus in patients with type 1 diabetes mellitus
PlosOne
11 October 2016
Yue J Wang
Materials
20 islets human donors
Number of markers
24 markers
First mass cytometry study on diabete in humans. Findings showing that mass cytometry can be used to analyze beta cells from pancreas
Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas
Cell Metabolism
31 October 2014
Michael Mingueneau
Materials
NOD mice
Number of markers
25 markers
TCR signaling dynamics in control C57BL/6 and autoimmunity-prone nonobese diabetic (NOD) mice. a small impairment in initial pCD3ζ activation resonates farther down the signaling cascade and results in larger defects in activation of the ERK1/2–S6 and IκBα modules.
Single-cell mass cytometry of TCR signaling: amplification of small initial differences results in low ERK activation in NOD mice
PNAS