Mass cytometry papers in diabetes (from the start of CyTOF till now)

You will find the list of papers of mass cytometry (CyTOF) investigating diabetes.

 

We also propose other reviews, one about mass cytometry data in blood cancers, the other in solid tumors. You might also be interested by the papers using mass cytometry to study COVID. There is a recent review about mass cytometry and type 1 diabetes. 

13 November 2020

Diance C. Saunders

Materials

Human tissues

Number of markers

Variable

Online imaging resource containing datasets linking imaging data with clinical data to facilitate advances in the understanding of diabetes, pancreatitis, and pancreatic cancer

Pancreatlas: Applying an Adaptable Framework to Map the Human Pancreas in Health and Disease

Patterns

Materials

Number of markers

15 June 2020

Martin Eichmann

Materials

PBMC from 59 individuals (placebo n = 19, abatacept n = 40)

Number of markers

32 markers

Mass cytometry showed significant association between TCM and β-cell function. Abatacept treatment significantly alters the frequencies of a majority of CD4+ conventional and regulatory T cell subsets but not CD8+ T cells.

Costimulation Blockade Disrupts CD4 + T Cell Memory Pathways and Uncouples Their Link to Decline in β-Cell Function in Type 1 Diabetes

Journal of Immunology

21 February 2020

Louise Magnusson

Materials

44 PBMC (including 15 healthy donors)

Number of markers

32 markers

Mass cytometry comparison of PBMC from patients with new-onset (N-T1D) and long-standing type 1 diabetes, Hashimoto's thyroiditis (HT), Graves' disease and autoimmune Addison's disease (AD), as well as healthy controls. CD56dimCD69+HLA-DR- NK cells were reduced and CD8+ TEMRA cells were reduced in the N-T1D group.

Mass Cytometry Studies of Patients With Autoimmune Endocrine Diseases Reveal Distinct Disease-Specific Alterations in Immune Cell Subsets

Frontiers in Immunology

02 January 2020

Alice E Wiedeman

Materials

66 islets human including 20 controls

Number of markers

22 markers

Study of the phenotype and function of autoreactive CD8+ T cells. influence disease progression with application of new bioinformatic method DISCOV-R. Activated islet-specific CD8+ memory T cells were prevalent in subjects with T1D who experienced rapid loss of C-peptide.

Autoreactive CD8+ T cell exhaustion distinguishes subjects with slow type 1 diabetes progression

Journal of Clinical Investigation

24 November 2019

Jessica Suwandi

Materials

PBMC and in vitro stimulation

Number of markers

35 markers

Identification of a surface-based T cell signature of tolerogenic modulation (specifically tolerogenic dendritic cells, Tol DCs) by CyTOF. TolDCs induced suppressive T cells mostly CD45RA+CCR7+. T cell cultures stimulated by tolDC further contained memory-like CD45RA-CCR7- T cells expressing regulatory markers Lag-3, CD161 and ICOS.

Multidimensional analyses of proinsulin peptide-specific regulatory T cells induced by tolerogenic dendritic cells

Journal of Automimmunity

03 May 2019

Hugo Barcenilla

Materials

9 high risk individuals versus 9 controls

Number of markers

33 markers

CyTOF identified an effector memory Treg subset expressing HLA-DR, CCR4, CCR6, CXCR3, and GATA3 that was increased in the high-risk group. for type 1 diabetes. This was correlated also with increase of other NK subsets defined by CD16+ CD8+ CXCR3+ and CD16+ CD8+ CXCR3+ CD11c+. These markers might be predictive of type 1 diabetes

Mass Cytometry Identifies Distinct Subsets of Regulatory T Cells and Natural Killer Cells Associated With High Risk for Type 1 Diabetes

Frontiers in Immunology

05 March 2019

Nicolas Damond

Materials

12 islets human donors

Number of markers

35 markers

Analysis of 1,581 islets from 12 human donors (8 with type 1 diabete), using imaging mass cytometry (IMC). Pseudotime analysis of islets through T1D progression from snapshot data to reconstruct the evolution of β cell loss and insulitis revealed that β cell destruction is preceded by a β cell marker loss and by recruitment of cytotoxic and helper T cells.

A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry

Cell Metabolism

05 March 2019

Yue J Wang

Materials

12 islets T1D donors and 6 controls

Number of markers

33 markers

Islet cells show changes in protein expression during T1D progression and immune cells are higly proliferative in type 1 diabetes

Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes

Cell Metabolism

08 August 2018

Sandra Laban

Materials

3 islet type 1 diabetes patients

Number of markers

33 markers

Mass cytometry description of autoantigen- (pre-proinsulin), neoantigen- (insulin-DRIP) and virus- (cytomegalovirus) reactive CD8 T-cells.

Heterogeneity of circulating CD8 T-cells specific to islet, neo-antigen and virus in patients with type 1 diabetes mellitus

PlosOne

11 October 2016

Yue J Wang

Materials

20 islets human donors

Number of markers

24 markers

First mass cytometry study on diabete in humans. Findings showing that mass cytometry can be used to analyze beta cells from pancreas

Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas

Cell Metabolism

31 October 2014

Michael Mingueneau

Materials

NOD mice

Number of markers

25 markers

TCR signaling dynamics in control C57BL/6 and autoimmunity-prone nonobese diabetic (NOD) mice. a small impairment in initial pCD3ζ activation resonates farther down the signaling cascade and results in larger defects in activation of the ERK1/2–S6 and IκBα modules.

Single-cell mass cytometry of TCR signaling: amplification of small initial differences results in low ERK activation in NOD mice

PNAS

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