Mass cytometry papers in COVID from 2020

Investigation of transmembrane proteins in platelets of hospitalized COVID-19 patients. Non-stimulated platelets compared to stimulated platelets. In COVID patients, significantly reduced capacity of platelets to increase the expression of activation markers LAMP-3 and P-Selectin upon stimulation with TRAP.

Comparison of peripheral blood between HD and COVID patients and identification of CD4 depletion.

Characterisation of T cells from COVID19 recovered patients. SARS-CoV-2-specific CD4+ T cells were exclusively Th1 cells (predominantly Tcm). SARS-CoV-2-
specific CD8+ T cells were predominantly TemRA cells (in less terminal differentiation state). Subsets of SARS-CoV-2-specific T cells express CD127, can homeostatically proliferate, and can persist for over two months.

Due to differential immune cell profile of cancer patients who are treated with immunomodulatory agents, the host response to the SARS-COV2 may lessen symptom severity.

Characterisation of the immune response to COVID19 over time in the blood. IFNγ-eosinophil axis activated before lung hyperinflammation

Analysis of immune responses in between COVID-19 patients and healthy individuals from Hong Kong and Atlanta

Combined scRNA-seq, mass cytometry and scATAC-seq to compare immune cell types in peripheral blood collected from young and old subjects and patients with COVID-19

Integration of multiple platforms to study COVID19 over time showing:
- Mild COVID-19 corrolates with HLA-DRhiCD11chi CD14+ monocytes
- Severe COVID-19 corrolates with HLA-DRloCD163hi and HLA-DRloS100Ahi CD14+ monocytes

Mass cytometry to investigate peripheral myeloid cells and T lymphocytes from 30 convalescent patients with mild, moderate, and severe cases of COVID-19. CD14+CD16+ intermediate and CD14dimCD16+ nonclassical monocytes, as well as CD4+ stem cell memory T (TSCM) cells, correlated with COVID-19 severity. Both intermediate and non-classical monocyte subsets shape the adaptive immune response to SARS-CoV-2

Highly impaired IFN response (characterized by no IFN-β and low IFN-α production and activity) was found to be associated with a persistent blood viral load. Type-I IFN deficiency in blood could be a hallmark of severe COVID-19.

Validation of a workflow for staining of whole blood samples with minimal processing requirements or expertise at the site of sample collection, followed by shipment to a central CyTOF core facility for batched downstream processing and data acquisition. Characterization of immune responses of COVID-19 patients, highlighting key disease-associated changes in immune cell frequency and phenotype.

Mass cytometry to study COVID-19 patients. Good prognoses had significantly higher counts of monocytes, macrophages, higher frequency of CD3+CD4+CD45RO+CXCR3+ subsets, higher frequency of CD14+CD11C+HLA‐DR+ subset of dendritic cells and a lower count of neutrophils. Proportions of naïve CD4+ T cells, Tregs, and Th2 cells in the poor prognosis group were relatively higher than those in the good prognosis group, and CD4+ memory T cells were relatively lower.

Mass cytometry profiled immune cellular components to analyze the peripheral blood mononuclear cells (PBMCs) from patients with differences in disease progression by comparing with the PBMCs from healthy donors (HDs).

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